Dual-laser fluorescence correlation spectroscopy as a biophysical probe of binding interactions: evaluation of new red fluorescent dyes

Kerry M. Swift; Steven Anderson; Edmund D. Matayoshi

doi:10.1117/12.426726

10 May 2001 Dual-laser fluorescence correlation spectroscopy as a biophysical probe of binding interactions: evaluation of new red fluorescent dyes

Kerry M. Swift, Steven Anderson, Edmund D. Matayoshi

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Proceedings Volume 4252, Advances in Fluorescence Sensing Technology V; (2001) https://doi.org/10.1117/12.426726
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

In this work we characterized seven dyes that are one-photon excited and fluoresce in the far-red region of the visible spectrum, five of which became available only recently, as to their suitability as labels on the red emission channel in a two-color FSC method. Spectroscopic properties and binding to albumin were studied. In addition, when one of these was used as the label in a first-pass high-throughput screen with single-color fluorescence polarization detection, its foreseen advantage of avoiding the excitation of interfering background autofluorescence from compounds in libraries was deemed valuable after a comparison of two complete high-throughput screens of a chemical compound library at Abbott.

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Kerry M. Swift, Steven Anderson, and Edmund D. Matayoshi "Dual-laser fluorescence correlation spectroscopy as a biophysical probe of binding interactions: evaluation of new red fluorescent dyes", Proc. SPIE 4252, Advances in Fluorescence Sensing Technology V, (10 May 2001); https://doi.org/10.1117/12.426726

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