Fluorescence lifetime attachment LIFA

Cornelius J. R. van der Oord; Karel W. J. Stoop; Lambertus K. van Geest

doi:10.1117/12.426731

10 May 2001 Fluorescence lifetime attachment LIFA

Cornelius J. R. van der Oord, Karel W. J. Stoop, Lambertus K. van Geest

Proceedings Volume 4252, Advances in Fluorescence Sensing Technology V; (2001) https://doi.org/10.1117/12.426731
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

We present the Lambert Instruments Fluorescence Lifetime Attachment LIFA. LIFA enables easy to use and affordable microscopy and macroscopic FLIM. The system implements the homodyne detection scheme for measuring the fluorescence lifetime in each pixel of the image. The microscopy system features an ultra bright LED illuminator, the LI-(mu) Cam intensified CCD camera a high frequency signal generator. The illuminator replaces the excitation light source of a standard fluorescence microscopy, while the LI-(mu) CAM intensified CCD camera is attached to the photo-port. Both the illuminator and the intensifier are modulated at a frequency up to 100 MHz at a series of phase differences. The lifetime image is calculated from the series of images on a personal computer.

Citation Download Citation

Cornelius J. R. van der Oord, Karel W. J. Stoop, and Lambertus K. van Geest "Fluorescence lifetime attachment LIFA", Proc. SPIE 4252, Advances in Fluorescence Sensing Technology V, (10 May 2001); https://doi.org/10.1117/12.426731

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