10 May 2001 Fluorescence lifetime attachment LIFA
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Proceedings Volume 4252, Advances in Fluorescence Sensing Technology V; (2001) https://doi.org/10.1117/12.426731
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States
Abstract
We present the Lambert Instruments Fluorescence Lifetime Attachment LIFA. LIFA enables easy to use and affordable microscopy and macroscopic FLIM. The system implements the homodyne detection scheme for measuring the fluorescence lifetime in each pixel of the image. The microscopy system features an ultra bright LED illuminator, the LI-(mu) Cam intensified CCD camera a high frequency signal generator. The illuminator replaces the excitation light source of a standard fluorescence microscopy, while the LI-(mu) CAM intensified CCD camera is attached to the photo-port. Both the illuminator and the intensifier are modulated at a frequency up to 100 MHz at a series of phase differences. The lifetime image is calculated from the series of images on a personal computer.
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Cornelius J. R. van der Oord, Cornelius J. R. van der Oord, Karel W. J. Stoop, Karel W. J. Stoop, Lambertus K. van Geest, Lambertus K. van Geest, } "Fluorescence lifetime attachment LIFA", Proc. SPIE 4252, Advances in Fluorescence Sensing Technology V, (10 May 2001); doi: 10.1117/12.426731; https://doi.org/10.1117/12.426731
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