Multiphoton-excited fluorescence particle metrology:  application to silica hydrogels

David J. S. Birch; Chris D. Geddes; Jan Karolin; Klaas Wynne

doi:10.1117/12.426737

10 May 2001 Multiphoton-excited fluorescence particle metrology: application to silica hydrogels

David J. S. Birch, Chris D. Geddes, Jan Karolin, Klaas Wynne

Proceedings Volume 4252, Advances in Fluorescence Sensing Technology V; (2001) https://doi.org/10.1117/12.426737
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

We demonstrate a new application of multiphoton excitation of fluorescence; the measurement of silica particle growth during sol-gel polymerization. Recently we have reported the use of fluorescence anisotropy as an alternative approach to nm particle sizing using conventional techniques such as small angle light, neutron or x-ray scattering. Advantages of our approach include near angstrom resolution, minimal interference from the gel network as well as the additional benefits of providing microviscosity and hydrodynamic information. Fluorescence anisotropy applied to particle sizing in colloids in general is still in its infancy, but in this paper we show using probes fluorescein and rhodamine 6G, under conditions unsuitable for one-photon excitation, can be successful with multiphoton excitation.

Citation Download Citation

David J. S. Birch, Chris D. Geddes, Jan Karolin, and Klaas Wynne "Multiphoton-excited fluorescence particle metrology: application to silica hydrogels", Proc. SPIE 4252, Advances in Fluorescence Sensing Technology V, (10 May 2001); https://doi.org/10.1117/12.426737

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