Application of diode laser/fiber optic colorimetric spectrometer to the determination of cholesterol

SungHo Kim; Shin-Young Yun; Jeong-Hwa Lee; Hyo-Young Lee; Hae-Seon Nam

doi:10.1117/12.426744

22 May 2001 Application of diode laser/fiber optic colorimetric spectrometer to the determination of cholesterol

SungHo Kim, Shin-Young Yun, Jeong-Hwa Lee, Hyo-Young Lee, Hae-Seon Nam

Proceedings Volume 4255, Clinical Diagnostic Systems; (2001) https://doi.org/10.1117/12.426744
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

A simple and inexpensive colorimetric spectrometer for determining total cholesterol has been developed, comprising a diode laser as the light source, optical fibers for the light guide and a photodiode as the detector. In order to generate the color change, enzymatic reaction of cholesterol oxidase-peroxidase method was used. The stability and performance of the new system was investigated by obtaining the calibration curve for standard cholesterol solutions. The total cholesterol in human serum was also measured by the analyzer and compared with the value obtained by a conventional chemistry analyzer. The results showed that the developed spectrometer was useful for the determination of cholesterol levels.

Citation Download Citation

SungHo Kim, Shin-Young Yun, Jeong-Hwa Lee, Hyo-Young Lee, and Hae-Seon Nam "Application of diode laser/fiber optic colorimetric spectrometer to the determination of cholesterol", Proc. SPIE 4255, Clinical Diagnostic Systems, (22 May 2001); https://doi.org/10.1117/12.426744

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