22 May 2001 Novel high-sensitivity fluorescence polarization reader
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Proceedings Volume 4255, Clinical Diagnostic Systems; (2001) https://doi.org/10.1117/12.426755
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States
Abstract
We have developed a new fluorescence polarization (FP) reader suitable for high-throughput screening (HST) and ultra-HTS whose assay-performance and sample-throughput are both considerably improved over present state-of-the-art instrumentation. The SymmetryTM reader possesses a number of features that differ from conventional HTS FP readers. These include: laser-based excitation, liquid crystal polarization optics that rapidly and accurately measure polarization states; and CCD detectors to capture emission from multiple wells. We show that the performance in assays relevant to the drug discovery process, such as G- protein coupled receptor-based assays, is significantly enhanced due to a dramatic improvement in precision. Furthermore, the CCD-detection system used can substantially improve sample throughput compared to sequential readers while maintaining high performance.
© (2001) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Clifford C. Hoyt, Clifford C. Hoyt, Richard M. Levenson, Richard M. Levenson, Peter Banks, Peter Banks, "Novel high-sensitivity fluorescence polarization reader", Proc. SPIE 4255, Clinical Diagnostic Systems, (22 May 2001); doi: 10.1117/12.426755; https://doi.org/10.1117/12.426755
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