15 June 2001 Optoacoustic control system for selective treatment of the retinal pigment epithelium
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Proceedings Volume 4256, Biomedical Optoacoustics II; (2001) https://doi.org/10.1117/12.429323
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States
Abstract
The selective damage of the retinal pigment epithelium (RPE) is a new treatment method for several retinal diseases. By applying a train of microsecond(s) laser pulses it is possible to selectively damage these cells and simultaneously spare the adjacent photoreceptor and neural tissue. Due to the ophthalmologic invisibility of the RPE cell damage we investigate an optoacoustic (OA) control system to monitor the RPE cell damage. Setup: The irradiation was performed with a frequency doubled Nd:YLF laser by applying a train of +s laser pulses. In vitro, the OA transients were received by an ultrasonic broadband transducer. During treatment an OA contact lens with embedded transducer was used. In vitro: Freshly enucleated porcine RPE samples with CalceinAM as life/death staining were used. Below RPE cell damage threshold a classic thermoelastic transient was found. Above cell damage threshold the OA transient differs form pulse to pulse. This can be explained by microbubble formation around the strong absorbing melanosomes inside the RPE cells. In vivo: We found the same pulse to pulse deviations of the OA transient above the fluoresceine angiographic detectable RPE damage threshold during treatment. This system give us a new approach to non-invasively monitor the selective RPE treatment.
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Georg Schuele, Elke Joachimmeyer, Carsten Framme, Johann Roider, Reginald Birngruber, Ralf Brinkmann, "Optoacoustic control system for selective treatment of the retinal pigment epithelium", Proc. SPIE 4256, Biomedical Optoacoustics II, (15 June 2001); doi: 10.1117/12.429323; https://doi.org/10.1117/12.429323
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