9 July 2001 Wavelength-dependent scattering of light during Nd:YAG laser heating of porcine septal cartilage
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Proceedings Volume 4257, Laser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical; (2001) https://doi.org/10.1117/12.434728
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States
Abstract
Heat alters the bulk physical properties of cartilage tissue, including the optical scattering and absorption coefficients. The purpose of this investigation was to measure wavelength dependent scattering of light using three different probe lasers (λ= 488 nm, 670 nm, 808 nm) during Nd:YAG laser (λ= 1.32 micrometers , 50 Hz pulse repetition rate) heating of porcine septal cartilage. An integrating sphere was used to collect diffusely backscattered light from these probe lasers and three lock- in amplifiers were used to discriminate between the different signals. Peak signal intensity of the backscattered light was observed at different temperature depending on the probe laser wavelength and specimen thickness. The observed changes are unlikely due to axial thermal gradients created during Nd:YAG laser heating and do not correlate with the fluence distribution of the three probe laser wavelengths evaluated. The observed wavelength dependent differences suggest that tissue matrix alterations during heating are due to macromolecular conformation changes that occur on the scale of the wavelength of the probe laser light. As changes in the bulk properties of cartilage can be inferred by using simple non-contact techniques such as light scattering, the characterization of the wavelength dependence of these phenomena will become increasingly important.
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Reshmi Basu, Reshmi Basu, Brian Jet-Fei Wong, Brian Jet-Fei Wong, Steen J. Madsen, Steen J. Madsen, } "Wavelength-dependent scattering of light during Nd:YAG laser heating of porcine septal cartilage", Proc. SPIE 4257, Laser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical, (9 July 2001); doi: 10.1117/12.434728; https://doi.org/10.1117/12.434728
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