Flow cytometer using a fiber optic detection system

Masahiko Kanda; Motomi Nakata; Misako Osoegawa; Shinichirou Niwa; Tatsuo Yamashita; Shingo Suzuki; Koji Murayama

doi:10.1117/12.426767

10 May 2001 Flow cytometer using a fiber optic detection system

Masahiko Kanda, Motomi Nakata, Misako Osoegawa, Shinichirou Niwa, Tatsuo Yamashita, Shingo Suzuki, Koji Murayama

Proceedings Volume 4260, Optical Diagnostics of Living Cells IV; (2001) https://doi.org/10.1117/12.426767
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

A flow cytometer with a new hybrid flow cell has been developed. The hybrid flow cell was constructed closed-type quarts curvets which produces a parabolic profile of flow velocities. It was involved a fiber-optic detecting unit for a high sensitivity collection and had a jet nozzle and a PZT device for droplet formation aimed to cell sorting. A optical fiber that consists of a glass core and have a core diameter of 200 micrometers with NA of 0.5 have been successfully used for the collection of side scatter and fluorescent signals. Two detecting fibers mounted in a hybrid flow cell with pith of 250 micrometers are designed to individually detect fluorescent signals excited by 2 lasers, which are a compact air-cooled AR laser (488 nm: 20 mW) and a semiconductor laser (640 nm: 15 mW). As a result, a sensitivity of our flow cytometer is 300 MESF determined using fluorescein-labeled beads and 2 scatter and 6 fluorescent signals could successfully be detected. Typical patterns of cell cycle were observed with Daudi human Burkitt Lymphoma cell lines stained with PL. Furthermore, the alignment requirements are more simplified because of a hybrid flow cell stably mounted.

Citation Download Citation

Masahiko Kanda, Motomi Nakata, Misako Osoegawa, Shinichirou Niwa, Tatsuo Yamashita, Shingo Suzuki, and Koji Murayama "Flow cytometer using a fiber optic detection system", Proc. SPIE 4260, Optical Diagnostics of Living Cells IV, (10 May 2001); https://doi.org/10.1117/12.426767

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