Point spread functions for particle imaging using inhomogeneous polarization in scanning optical microscopy

Kathleen S. Youngworth; David P. Bliss; Thomas G. Brown

doi:10.1117/12.424532

16 April 2001 Point spread functions for particle imaging using inhomogeneous polarization in scanning optical microscopy

Kathleen S. Youngworth, David P. Bliss, Thomas G. Brown

Author Affiliations +

Proceedings Volume 4261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VIII; (2001) https://doi.org/10.1117/12.424532
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Polarization control and analysis continue to be a useful tools for microscopy. We have previously addressed the use of spatially inhomogeneous polarization, specifically azimuthal polarization, in scanning optical microscopy. In this paper we describe new techniques for generating both azimuthally and radially polarized cylindrical-vector beams, investigate high numerical aperture focusing, compare the theoretical and experimental point spread functions for small particle imaging, and explore the use of these beams with microscopy.

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Kathleen S. Youngworth, David P. Bliss, and Thomas G. Brown "Point spread functions for particle imaging using inhomogeneous polarization in scanning optical microscopy", Proc. SPIE 4261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VIII, (16 April 2001); https://doi.org/10.1117/12.424532

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