16 April 2001 Three-dimensional imaging with a quadrature tomographic microscope
Author Affiliations +
Proceedings Volume 4261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VIII; (2001) https://doi.org/10.1117/12.424533
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States
Abstract
he quadrature tomographic microscope combines conventional microscopy with interferometric quadrature detection techniques to yield a simultaneous measurement of both the amplitude and phase of the electric field. This is of tremendous utility in imaging nearly-transparent object's since changes in phase can be directly attributed to changes in the objects index of refraction while the small amplitude changes provide additional structural information. In this paper we will discuss the progress we have made towards reconstructing three dimensional images from the measured two-dimensional complex electric field. By applying an artificial curvature to the measured field and back-propagating the Gaussian beams, we are able to digitally manipulate the imaging kernel and image different focal planes.
© (2001) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Johnathan J. Stott, Johnathan J. Stott, R. Eric Bennett, R. Eric Bennett, Carol M. Warner, Carol M. Warner, Charles A. DiMarzio, Charles A. DiMarzio, } "Three-dimensional imaging with a quadrature tomographic microscope", Proc. SPIE 4261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VIII, (16 April 2001); doi: 10.1117/12.424533; https://doi.org/10.1117/12.424533
PROCEEDINGS
9 PAGES


SHARE
Back to Top