24 April 2001 Multisite two-photon imaging of neurons on multielectrode arrays
Author Affiliations +
Proceedings Volume 4262, Multiphoton Microscopy in the Biomedical Sciences; (2001) https://doi.org/10.1117/12.424542
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States
We wish to understand how neural systems store, recall, and process information. We are using cultured networks of cortical neurons grown on microelectrode arrays as a model system for studying the emergent properties of ensembles of living neurons. We have developed a 2-way communication interface between the cultured network and a computer- generated animal, the Neurally Controlled Animat. Neural activity is used to control the behavior of the Animat, and 2- photon time-lapse imaging is carried out in order to observe the morphological changes that might underlie changes in neural processing. The 2-photon microscope is ideal for repeated imaging over hours or days, with submicron resolution and little photodamage. We have designed a computer-controlled microscope stage that allows imaging several locations in sequence, in order to collect more image data. For the latest progress, see: http://www.caltech.edu/~pinelab/PotterGroup.htm.
© (2001) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Steve M. Potter, Steve M. Potter, Natalia Lukina, Natalia Lukina, Kenneth J. Longmuir, Kenneth J. Longmuir, Yan Wu, Yan Wu, } "Multisite two-photon imaging of neurons on multielectrode arrays", Proc. SPIE 4262, Multiphoton Microscopy in the Biomedical Sciences, (24 April 2001); doi: 10.1117/12.424542; https://doi.org/10.1117/12.424542

Back to Top