Nonlinear multimodality spectromicroscopy: multiphoton fluorescence, SHG, and THG of biological specimen

Ping Chin Cheng; Chi-Kuang Sun; Bai-Ling Lin; Fu-Jen Kao; Shi-Wei Chu

doi:10.1117/12.424541

24 April 2001 Nonlinear multimodality spectromicroscopy: multiphoton fluorescence, SHG, and THG of biological specimen

Ping Chin Cheng, Chi-Kuang Sun, Bai-Ling Lin, Fu-Jen Kao, Shi-Wei Chu

Proceedings Volume 4262, Multiphoton Microscopy in the Biomedical Sciences; (2001) https://doi.org/10.1117/12.424541
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

The non-linear nature of multi-photon fluorescence excitation, SHG and THG restricts the signal detecting volume to the vicinity of the focal point. As a result, the technology has intrinsic optical sectioning capability. The use of multi-photon fluorescence excitation also allows micro-fluorometry at high spatial resolution. Under high intensity illumination, biological specimen not only emits fluorescence, but also generates harmonic emissions. Conventional ultra-fast Ti-sapphire laser allows efficient excitation of most biologically important fluorescent probes and SHG in the deep blue range. In contrast, the use of ultra-fast Cr-forsterite laser makes possible simultaneous detection of two- and three-photon fluorescence, SHG and THG

Citation Download Citation

Ping Chin Cheng, Chi-Kuang Sun, Bai-Ling Lin, Fu-Jen Kao, and Shi-Wei Chu "Nonlinear multimodality spectromicroscopy: multiphoton fluorescence, SHG, and THG of biological specimen", Proc. SPIE 4262, Multiphoton Microscopy in the Biomedical Sciences, (24 April 2001); https://doi.org/10.1117/12.424541

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