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24 April 2001 Practical guide for constructing a pulse compressor used in multiphoton microscopy
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Proceedings Volume 4262, Multiphoton Microscopy in the Biomedical Sciences; (2001)
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States
Multi-photon imaging has generated intense interest from investigators using fluorescent imaging assays in live cells. It can be argued that this technique is rapidly overtaking confocal microscopy as the method of choice for three- dimensional fluorescent imaging of in vivo preparations. Because of the cost involved in purchasing a commercial multi- photon imaging microscope, many investigators have elected to build their own system by adapting in-house confocal laser scanning microscopes. One of the components used for this adaptation involves the construction of an external pulse compressor. Pulse compressors are used to add negative dispersion to pulsed radiation, which undergoes group velocity dispersion when traversing optical elements. In this chapter, we describe a practical guide to building an external pulse compressor.
© (2001) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
James D. Lechleiter, Da-Ting Lin, and Ilse Sienaert "Practical guide for constructing a pulse compressor used in multiphoton microscopy", Proc. SPIE 4262, Multiphoton Microscopy in the Biomedical Sciences, (24 April 2001);


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