Joint optimization of lighting and sensing for the CCD-type DNA chip scanner

Jong Dae Kim; Jin-Hee Kim; Jongwon Kim

doi:10.1117/12.424588

16 April 2001 Joint optimization of lighting and sensing for the CCD-type DNA chip scanner

Jong Dae Kim; Jin-Hee Kim; Jongwon Kim

Author Affiliations +

Proceedings Volume 4264, Genomics and Proteomics Technologies; (2001) https://doi.org/10.1117/12.424588
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

While the DNA chip is going to be prevalent for the medical diagnostics and drug development, its analysis tool such as a chip scanner need to be optimized. To obtain the guideline for the optimized CCD-based scanner, this paper addresses the minimum requirements for the selected CCD-based detector under the chosen light condition. The scope of our experiments was focused on the scanning performance rather than the design of the chip scanner itself. Through the gray level analysis of the target spot and background, we could check the possibility of the CCD-based detector and the lighting lamp to adopt them as a low price scanner. A quality measure was investigated with two levels of lighting power and the various integration times. For the CCD-based detector and the Xenon lighting system, we evaluated the image performance with the developed quality measure. We define a background and a sample area mask manually to investigate the statistics of the images captured by the scanner. The developed system is composed with a medium price CCD camera and a zenon lamp lighting system. The developed system has reasonable performance for the scanned image. The risk ofphotobleaching was not shown in our experiments.

Citation Download Citation

Jong Dae Kim, Jin-Hee Kim, Jongwon Kim, "Joint optimization of lighting and sensing for the CCD-type DNA chip scanner", Proc. SPIE 4264, Genomics and Proteomics Technologies, (16 April 2001); doi: 10.1117/12.424588; https://doi.org/10.1117/12.424588

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