Solution of the inverse light-scattering problem for suspension of spherical biological particles: application in scanning flow cytometry

Nickolay V. Shepelevich; Inna V. Prostakova; Valeriy N. Lopatin

doi:10.1117/12.411994

29 December 2000 Solution of the inverse light-scattering problem for suspension of spherical biological particles: application in scanning flow cytometry

Nickolay V. Shepelevich, Inna V. Prostakova, Valeriy N. Lopatin

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Proceedings Volume 4341, Seventh International Symposium on Atmospheric and Ocean Optics; (2000) https://doi.org/10.1117/12.411994
Event: 7th International Symposium on Atmospheric and Ocean Optics, 2000, Tomsk, Russian Federation

Abstract

The relations between intensities of light scattering indicatrix and relative refractive index of a homogeneous sphere have been studied analytically and numerically for large optically 'soft' particles. The regions where solution of inverse problem is available have been determined. The obtained results reveal the opportunity of determination of relative refractive index for individual and suspension of spherical particles from experimental indicatrix.

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Nickolay V. Shepelevich, Inna V. Prostakova, and Valeriy N. Lopatin "Solution of the inverse light-scattering problem for suspension of spherical biological particles: application in scanning flow cytometry", Proc. SPIE 4341, Seventh International Symposium on Atmospheric and Ocean Optics, (29 December 2000); https://doi.org/10.1117/12.411994

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