14 September 2001 Direct electrochemistry of pentachlorophenol hydroxylase
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Proceedings Volume 4414, International Conference on Sensor Technology (ISTC 2001); (2001); doi: 10.1117/12.440184
Event: International Conference on Sensing units and Sensor Technology, 2001, Wuhan, China
Abstract
The direct electrochemistry of flavin-containing monooxygenase, pentachlorophenol hydroxlase (PCPH), has been investigated under a variety of conditions. PCPH underwent a two-electron process on the electrodes, which correspond to the reduction/oxidation of FAD/FADH2 within the enzyme. The electrochemical response of PCPH relies on the interface of the electrode and the enzyme solution. Three types of interaction of the enzyme molecule with the electrodes were observed: at a bare edge-plane graphite electrode, diffusion-controlled process was observed, suggesting the interaction is weak. In the presence of the cations, the interaction became stronger, so that the voltammetric response changed from a diffusion control to an adsorption control An intermediate case was observed at a poly(L- lysine) modified EPG electrode.
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Wei-hong Xie, H. Allen, O. Hill, Luet L. Wong, "Direct electrochemistry of pentachlorophenol hydroxylase", Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); doi: 10.1117/12.440184; https://doi.org/10.1117/12.440184
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