14 September 2001 Fiber optic biosensor using aptamer as receptors
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Proceedings Volume 4414, International Conference on Sensor Technology (ISTC 2001); (2001) https://doi.org/10.1117/12.440213
Event: International Conference on Sensing units and Sensor Technology, 2001, Wuhan, China
Reagentless biosensor that can directly transducer molecular recognition to optical signal should potentiate the development of sensor array fora wide variety of analytes. Nucleic acid aptamer can bind ligand tightly and specifically with conformational change of aptamer, and can be used as a receptor in biosensor. We have therefore developed a fiber-optic biosensor by aptamer connected with molecular beacon. Molecular beacons consist of an oligonucleotide sequence containing complementary sequence sections at either end. These two sequence containing segments base pair with each other to form a hairpin shaped loop structure, the fluorophore and quencher were attached at 5 foot- and 3 foot-end of molecular beacon respectively. When thrombin binding to the stem-loop of molecular beacon aptamer, the pseudoknot structure was interrupted, resulting a release of fluorescence from quenching and a increase in fluorescence emission. This novel biosensor system in this project has a large potential and is specific and sensitivity. A similar strategy could be used to study other analytes such as protein and small molecules.
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Shuqin Yu, Shuqin Yu, Xiaokun Cai, Xiaokun Cai, Xianglin Tan, Xianglin Tan, Yexiang Zhu, Yexiang Zhu, Bin Lu, Bin Lu, } "Fiber optic biosensor using aptamer as receptors", Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); doi: 10.1117/12.440213; https://doi.org/10.1117/12.440213

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