14 September 2001 Fiber-optic evanescent wave biosensor of catecholamine neurotransmitter
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Proceedings Volume 4414, International Conference on Sensor Technology (ISTC 2001); (2001) https://doi.org/10.1117/12.440174
Event: International Conference on Sensing units and Sensor Technology, 2001, Wuhan, China
Abstract
Using quartz fiber-immobilized laccase, detection of catecholamine neurotransmitter is described in this work. Laccase is immobilized on the fiber-optic by means of 3- aminopropyltriethoxysilane/glutaraldehyde method. The oxidation products of adrenalin catalyzed by laccade would absorb the fiber-optic evanescent wave according to the products' concentration. The optimal detection range of this fiber-optic biosensor is between 50-250ng/ml. The minimum detection limit is 10ng/ml. The analysis can provide results in only two minutes to detect one sample. Finally, the specificity of the biosensor is high. The special interference of other substrates of laccase such as o- phyenylenediamine (OPD) and benzenediol can be removed by controlling the pH of the reaction buffer. When the OPD concentration is 100ng/ml, the relative error is only 6.3 percent. On the other hand, the non-special interference is removed by employing double-channel differential method.
© (2001) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Yexiang Zhu, Yong Ran, Shunqing Xu, "Fiber-optic evanescent wave biosensor of catecholamine neurotransmitter", Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); doi: 10.1117/12.440174; https://doi.org/10.1117/12.440174
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