Long synthetic oligonucleotides for microarray expression measurement

Jiong Li; Hong Wang; Heping Liu; M. Zhang; Chunxiu Zhang; Zu-Hong Lu; Xiang Gao; Dong Kong

doi:10.1117/12.440169

14 September 2001 Long synthetic oligonucleotides for microarray expression measurement

Jiong Li, Hong Wang, Heping Liu, M. Zhang, Chunxiu Zhang, Zu-Hong Lu, Xiang Gao, Dong Kong

Proceedings Volume 4414, International Conference on Sensor Technology (ISTC 2001); (2001) https://doi.org/10.1117/12.440169
Event: International Conference on Sensing units and Sensor Technology, 2001, Wuhan, China

Abstract

There are generally two kinds of DNA microarray used for genomic-scale gene expression profiling of mRNA: cDNA and DNA chip, but both of them suffer from some drawbacks. To meet more requirements, another oligonucleotide microarray with long was produced. This type of microarray had the advantages of low cost, minimal Cross-hybridization, flexible and easy to make, which is most fit for small laboratories with special purposes. In this paper, we devised different probes with different probe lengths, GC contents and gene positions to optimization the probe design. Experiments showed 70 mer probes are suitable for both sufficient sensitivity and reasonable costs. Higher G-C content produces stronger signal intensity thus better sensitivity and probes designed at 3 untranslated region of gene within the range of 300 pb should be best for both sensitivity and specificity.

Citation Download Citation

Jiong Li, Hong Wang, Heping Liu, M. Zhang, Chunxiu Zhang, Zu-Hong Lu, Xiang Gao, and Dong Kong "Long synthetic oligonucleotides for microarray expression measurement", Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); https://doi.org/10.1117/12.440169

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