Molecular recognition technology for detection of protein target by molecular beacon

Shunqing Xu; Xiaokun Cai; Xianglin Tan; Bin Lu

doi:10.1117/12.440170

14 September 2001 Molecular recognition technology for detection of protein target by molecular beacon

Shunqing Xu, Xiaokun Cai, Xianglin Tan, Bin Lu

Proceedings Volume 4414, International Conference on Sensor Technology (ISTC 2001); (2001) https://doi.org/10.1117/12.440170
Event: International Conference on Sensing units and Sensor Technology, 2001, Wuhan, China

Abstract

Until recently, molecular beacons were limited to detecting nucleic acid target. Here we present a new technology for detection of thrombin protein by aptamer-derived molecule beacon without any antibody. A aptamer anti-thrombin was used as a recognition molecule. To make aptamer molecular beacon, we constructed two DNA oligomers that are derived from the aptamer to thrombin. To one of the DNA oligomers that forms a hairpin structure, the fluorophore and quencher were attac3hed at the 5 foot and 3 foot ends, respectively. Specially in the presence of Tat or its peptides, but not in the presence of other proteins, the two ligometers undergo a conformational change to form a duplex that leads to relieving of fluorophore from the quencher, and thus a significant enhancement of the fluorescence of fluorescein was observed. A novel strategy for exploiting aptamers in the analysis of thrombin has been described. A similar strategy could be used to study other analytes such as proteins and small molecules.

Citation Download Citation

Shunqing Xu, Xiaokun Cai, Xianglin Tan, and Bin Lu "Molecular recognition technology for detection of protein target by molecular beacon", Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); https://doi.org/10.1117/12.440170

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