14 September 2001 Molecular recognition technology for detection of protein target by molecular beacon
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Proceedings Volume 4414, International Conference on Sensor Technology (ISTC 2001); (2001) https://doi.org/10.1117/12.440170
Event: International Conference on Sensing units and Sensor Technology, 2001, Wuhan, China
Abstract
Until recently, molecular beacons were limited to detecting nucleic acid target. Here we present a new technology for detection of thrombin protein by aptamer-derived molecule beacon without any antibody. A aptamer anti-thrombin was used as a recognition molecule. To make aptamer molecular beacon, we constructed two DNA oligomers that are derived from the aptamer to thrombin. To one of the DNA oligomers that forms a hairpin structure, the fluorophore and quencher were attac3hed at the 5 foot and 3 foot ends, respectively. Specially in the presence of Tat or its peptides, but not in the presence of other proteins, the two ligometers undergo a conformational change to form a duplex that leads to relieving of fluorophore from the quencher, and thus a significant enhancement of the fluorescence of fluorescein was observed. A novel strategy for exploiting aptamers in the analysis of thrombin has been described. A similar strategy could be used to study other analytes such as proteins and small molecules.
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Shunqing Xu, Shunqing Xu, Xiaokun Cai, Xiaokun Cai, Xianglin Tan, Xianglin Tan, Bin Lu, Bin Lu, } "Molecular recognition technology for detection of protein target by molecular beacon", Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); doi: 10.1117/12.440170; https://doi.org/10.1117/12.440170
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