Single- and dual- near-infrared fluorescent labeled nucleic acid conjugate for nucleic acid detection

Zhihong Lin; Meng Wu; Shu Ren; Michaela Arbter; Martin Boehmer; Vladimir Mirsky; Otto S. Wolfbeis

doi:10.1117/12.440165

14 September 2001 Single- and dual- near-infrared fluorescent labeled nucleic acid conjugate for nucleic acid detection

Zhihong Lin, Meng Wu, Shu Ren, Michaela Arbter, Martin Boehmer, Vladimir Mirsky, Otto S. Wolfbeis

Proceedings Volume 4414, International Conference on Sensor Technology (ISTC 2001); (2001) https://doi.org/10.1117/12.440165
Event: International Conference on Sensing units and Sensor Technology, 2001, Wuhan, China

Abstract

In this research, a near-IR fluorescent labeled nucleic acid conjugate for the nucleic acid detection was synthesized, and characterized preliminarily for the detection of the nucleic acid. Th conjugate combines the molecular recognition properties of the oligonucleotides with the near-IR fluorescence label PR646. Both single- and dual- labeled conjugates were studied for their hybridization with the complementary nucleic acid. The dual labeled conjugate has indicated that the self-quenching effect exists in ssDNA form while the fluorescence increases greatly after hybridization with the complementary nucleic acids. The time-resolved fluorescence was also studied.

Citation Download Citation

Zhihong Lin, Meng Wu, Shu Ren, Michaela Arbter, Martin Boehmer, Vladimir Mirsky, and Otto S. Wolfbeis "Single- and dual- near-infrared fluorescent labeled nucleic acid conjugate for nucleic acid detection", Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); https://doi.org/10.1117/12.440165

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