Protein-orientating structures: thiocyanate layers as support for immobilization of bioreceptors

P. M. Boltovets; N. S. Dyachenko; Boris A. Snopok; Yuri M. Shirshov; Ya. D. Lampeka

doi:10.1117/12.429722

12 June 2001 Protein-orientating structures: thiocyanate layers as support for immobilization of bioreceptors

P. M. Boltovets, N. S. Dyachenko, Boris A. Snopok, Yuri M. Shirshov, Ya. D. Lampeka

Proceedings Volume 4425, Selected Papers from the International Conference on Optoelectronic Information Technologies; (2001) https://doi.org/10.1117/12.429722
Event: International Conference on Optoelectronic Information Technologies, 2000, Vinnytsia, Ukraine

Abstract

Using surface plasmon resonance we studied how the ferment- substrate interaction between trypsin and its soybean inhibitor at gold surface depended on previous treatment of the gold surface with NaNCS (thiocyanate) and NaNCO. It was shown that protein adsorption appreciably increased after surface modification by thiocyanate and didn't show any increase after their treatment with NaNCO. In the case of NaNCS treatment protein retained its biological properties and formed an oriented monolayer.

Citation Download Citation

P. M. Boltovets, N. S. Dyachenko, Boris A. Snopok, Yuri M. Shirshov, and Ya. D. Lampeka "Protein-orientating structures: thiocyanate layers as support for immobilization of bioreceptors", Proc. SPIE 4425, Selected Papers from the International Conference on Optoelectronic Information Technologies, (12 June 2001); https://doi.org/10.1117/12.429722

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