2 November 2001 Application of fluorescence-interference microscope for monitoring of dry weight dynamics and drug distribution within living cells
Author Affiliations +
Abstract
The authors have proposed and tested the new type of the microscope with superimposed interference and fluorescence images of the living cell. The fluorescence image gives the information about the distribution of drugs in different parts of the cell. Interference microscopy technique allows implementing the quantitative analysis of a living transparent cell, for example, to measure dry cell weight and cell density spatial distribution. Interference microscopy makes possibility the observation cell vital activity at dynamics. Computer-aided processing of interferograms using phase shift technique, allows carrying out continuous (over 12 hours and more) monitoring of living cells quantitative parameters with time interval between measurements less than one minute. RMS error for dry weight of non-living fixed test object using this microscope is about 1%.
© (2001) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Gennady G. Levin, Gennady G. Levin, Theodor V. Bulygin, Theodor V. Bulygin, Eugene Kalinin, Eugene Kalinin, Gennady N. Vishnyakov, Gennady N. Vishnyakov, Irene V. Goryainova, Irene V. Goryainova, } "Application of fluorescence-interference microscope for monitoring of dry weight dynamics and drug distribution within living cells", Proc. SPIE 4431, Photon Migration, Optical Coherence Tomography, and Microscopy, (2 November 2001); doi: 10.1117/12.447441; https://doi.org/10.1117/12.447441
PROCEEDINGS
6 PAGES


SHARE
Back to Top