Characterization of the diffusion of fluorophores within microbial biofilms by flourescence correlation microscopy under two-photon excitation

E. Guiot; M. P. Fontaine-Aupart; R. Briandet; M. N. Bellon-Fontaine; P. Georges; A. Brun; F. Merola; B. Arrio

doi:10.1117/12.447419

2 November 2001 Characterization of the diffusion of fluorophores within microbial biofilms by flourescence correlation microscopy under two-photon excitation

E. Guiot, M. P. Fontaine-Aupart, R. Briandet, M. N. Bellon-Fontaine, P. Georges, A. Brun, F. Merola, B. Arrio

Author Affiliations +

Proceedings Volume 4431, Photon Migration, Optical Coherence Tomography, and Microscopy; (2001) https://doi.org/10.1117/12.447419
Event: European Conferences on Biomedical Optics, 2001, Munich, Germany

Abstract

The penetration and diffusion capabilities of fluorophores inside microbial biofilms of heterogeneous three-dimensional structure were studied by fluorescence correlation microscopy under two-photon excitation. The influence of the size and the surface charge of the fluorophores were investigated in order to understand the mechanisms involved in molecular diffusion inhibition occuring in biofilms such as electrostatic and steric interactions.

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E. Guiot, M. P. Fontaine-Aupart, R. Briandet, M. N. Bellon-Fontaine, P. Georges, A. Brun, F. Merola, and B. Arrio "Characterization of the diffusion of fluorophores within microbial biofilms by flourescence correlation microscopy under two-photon excitation", Proc. SPIE 4431, Photon Migration, Optical Coherence Tomography, and Microscopy, (2 November 2001); https://doi.org/10.1117/12.447419

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