Five-dimensional fluorescence microscopy

Stephen E. D. Webb; D. S. Elson; Jan Siegel; Sandrine Leveque-Fort; Y. Gu; Duncan Parsons-Karavassilis; Mary J. Cole; Paul M. W. French; M. John Lever; Leon O. D. Sucharov; Mark A. A. Neil; Rimas Juskaitis; Tony Wilson

doi:10.1117/12.447405

2 November 2001 Five-dimensional fluorescence microscopy

Stephen E. D. Webb, D. S. Elson, Jan Siegel, Sandrine Leveque-Fort, Y. Gu, Duncan Parsons-Karavassilis, Mary J. Cole, Paul M. W. French, M. John Lever, Leon O. D. Sucharov, Mark A. A. Neil, Rimas Juskaitis, Tony Wilson

Author Affiliations +

Proceedings Volume 4431, Photon Migration, Optical Coherence Tomography, and Microscopy; (2001) https://doi.org/10.1117/12.447405
Event: European Conferences on Biomedical Optics, 2001, Munich, Germany

Abstract

We report a whole-field fluorescence imaging microscope that combines 3-D spatial resolution by optical sectioning, using structured illumination, with fluorescence lifetime imaging and spectrally-resolved imaging. We show the potential of this technique in the elimination of common artefacts in fluorescence lifetime imaging and apply it to study the dependence of the lifetime on the emission wavelength in biological tissue.

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Stephen E. D. Webb, D. S. Elson, Jan Siegel, Sandrine Leveque-Fort, Y. Gu, Duncan Parsons-Karavassilis, Mary J. Cole, Paul M. W. French, M. John Lever, Leon O. D. Sucharov, Mark A. A. Neil, Rimas Juskaitis, and Tony Wilson "Five-dimensional fluorescence microscopy", Proc. SPIE 4431, Photon Migration, Optical Coherence Tomography, and Microscopy, (2 November 2001); https://doi.org/10.1117/12.447405

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