2 November 2001 Five-dimensional fluorescence microscopy
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We report a whole-field fluorescence imaging microscope that combines 3-D spatial resolution by optical sectioning, using structured illumination, with fluorescence lifetime imaging and spectrally-resolved imaging. We show the potential of this technique in the elimination of common artefacts in fluorescence lifetime imaging and apply it to study the dependence of the lifetime on the emission wavelength in biological tissue.
© (2001) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Stephen E. D. Webb, Stephen E. D. Webb, D. S. Elson, D. S. Elson, Jan Siegel, Jan Siegel, Sandrine Leveque-Fort, Sandrine Leveque-Fort, Y. Gu, Y. Gu, Duncan Parsons-Karavassilis, Duncan Parsons-Karavassilis, Mary J. Cole, Mary J. Cole, Paul M. W. French, Paul M. W. French, M. John Lever, M. John Lever, Leon O. D. Sucharov, Leon O. D. Sucharov, Mark A. A. Neil, Mark A. A. Neil, Rimas Juskaitis, Rimas Juskaitis, Tony Wilson, Tony Wilson, "Five-dimensional fluorescence microscopy", Proc. SPIE 4431, Photon Migration, Optical Coherence Tomography, and Microscopy, (2 November 2001); doi: 10.1117/12.447405; https://doi.org/10.1117/12.447405

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