5 February 2002 Cloning of the 16S ribosomal RNA gene of a psychrophilic bacterium from the Alaskan Fox Permafrost Tunnel
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Abstract
Extreme cold environments on Earth, such as polar regions or deep ocean harbor a variety of life forms that have developed unique molecular mechanisms that allow them not only to survive, but also to proliferate under hostile conditions. Such organisms are specially relevant to astrobiology studies because they help determine the environmental limits within which life can exist. They can also have a huge potential for biotechnological applications, because of the unique properties of their macromolecules. In this study we focused on a newly isolated bacterium from the Fox Permafrost Tunnel, FTR1, that grows anaerobically at +2 degree(s)C. We describe the molecular phylogenetic analysis of this microorganism, through the cloning, sequencing and analysis of its 16S ribosomal RNA gene. Our results suggests that FTR1 is a novel species belonging to the Carnobacterium genus.
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Damien Marsic, Elena V. Pikuta, Richard B. Hoover, Joseph D. Ng, "Cloning of the 16S ribosomal RNA gene of a psychrophilic bacterium from the Alaskan Fox Permafrost Tunnel", Proc. SPIE 4495, Instruments, Methods, and Missions for Astrobiology IV, (5 February 2002); doi: 10.1117/12.454764; https://doi.org/10.1117/12.454764
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