7 January 2002 High-resolution element mapping inside biological samples using fluorescence microtomography
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Abstract
Sample preparation for element analysis of many biological tissues is difficult to achieve and prone to introduce contamination. Using x-ray fluorescence element microtomography (XFEMT) the element distribution on a virtual section across the sample can be determined with a resolution in the micrometer range. Fluorescence microtomograms of two plant samples are shown, demonstrating the possibility to map physiologically relevant ions, trace elements, and heavy metal pollutants at the cellular level. Attenuation effects inside the plant are corrected by a self-consistent tomographic reconstruction technique.
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Christian G. Schroer, Christian G. Schroer, Boris Benner, Boris Benner, Til Florian Guenzler, Til Florian Guenzler, Marion Kuhlmann, Marion Kuhlmann, Bruno Lengeler, Bruno Lengeler, Walter H. Schroeder, Walter H. Schroeder, Arnd J. Kuhn, Arnd J. Kuhn, Alexandre S. Simionovici, Alexandre S. Simionovici, Anatoly A. Snigirev, Anatoly A. Snigirev, Irina Snigireva, Irina Snigireva, "High-resolution element mapping inside biological samples using fluorescence microtomography", Proc. SPIE 4503, Developments in X-Ray Tomography III, (7 January 2002); doi: 10.1117/12.452850; https://doi.org/10.1117/12.452850
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