We describe cytometric system based on optical microscope for cell optical and photothermal quantitative studies. The developed methods are aimed to provide live cell analysis including monitoring, type and state differentiation. The developed image cytometry system was applied to the studies of the blood cells (RBC, WBC). Cell populations were analyzed through the 22 cell parameters. For parameter control aid optimization special objects that model the cells have been used. The hardware and software that provides image acquisition, processing and analysis are designed on modular principle. The results of cell studies have proven that the combined analysis of the two or three images of cell yields more information on cell for cell diagnostics. The developed system in its hardware and software are complementary to the optical microscopy.
Dmitry Lapotko, Dmitry Lapotko,
"Multiparameter cell diagnostics through optical and photothermal image analysis", Proc. SPIE 4515, Light and Optics in Biomedicine, (11 July 2001); doi: 10.1117/12.432970; https://doi.org/10.1117/12.432970