11 July 2001 Multiparameter cell diagnostics through optical and photothermal image analysis
Author Affiliations +
Proceedings Volume 4515, Light and Optics in Biomedicine; (2001) https://doi.org/10.1117/12.432970
Event: Light and Optics in Biomedicine, 2000, Warsaw, Poland
We describe cytometric system based on optical microscope for cell optical and photothermal quantitative studies. The developed methods are aimed to provide live cell analysis including monitoring, type and state differentiation. The developed image cytometry system was applied to the studies of the blood cells (RBC, WBC). Cell populations were analyzed through the 22 cell parameters. For parameter control aid optimization special objects that model the cells have been used. The hardware and software that provides image acquisition, processing and analysis are designed on modular principle. The results of cell studies have proven that the combined analysis of the two or three images of cell yields more information on cell for cell diagnostics. The developed system in its hardware and software are complementary to the optical microscopy.
© (2001) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Dmitry Lapotko, Dmitry Lapotko, "Multiparameter cell diagnostics through optical and photothermal image analysis", Proc. SPIE 4515, Light and Optics in Biomedicine, (11 July 2001); doi: 10.1117/12.432970; https://doi.org/10.1117/12.432970


Image analysis for diagnostics in photonic switching
Proceedings of SPIE (February 28 1991)
Study of the effect of CCD camera noise on microscopic...
Proceedings of SPIE (October 23 2006)
Instrumentation for quantitative fluorescence microscopy
Proceedings of SPIE (January 31 1995)
Photothermal imaging
Proceedings of SPIE (January 31 1995)

Back to Top