Detection of calcium by a confocal laser scanning microscope in Na2SeO3-treated SW480 human colonic carcinoma cells

Haitao Wang; Yafeng Liu; Zhihong Zhang; Xiangliang Yang; Hui-Bie F. Xu

doi:10.1117/12.462530

12 April 2002 Detection of calcium by a confocal laser scanning microscope in Na₂SeO₃-treated SW480 human colonic carcinoma cells

Haitao Wang, Yafeng Liu, Zhihong Zhang, Xiangliang Yang, Hui-Bie F. Xu

Author Affiliations +

Proceedings Volume 4536, International Workshop on Photonics and Imaging in Biology and Medicine; (2002) https://doi.org/10.1117/12.462530
Event: International Workshop on Photonics and Imaging in Biology and Medicine, 2001, Wuhan, China

Abstract

A number of studies have demonstrated that perturbed cellular calcium homeostasis has been implicated in apoptosis. Some studies showed that selenium compounds were able to induce cell apoptosis. The main objective of this study is to evaluate effect of Na₂SeO₃ on intracellular Ca²⁺ levels in SW480 human colonic carcinoma cells. When SW480 cells were exposed to 25 to 100 micrometers ol/L Na₂SeO₃, we also found that Na₂SeO₃ was able to induce [Ca²⁺]_i, disruption of mitochondrial membrane potential ((Delta) (psi) m) in SW480 cells by using a confocal laser scanning microscope. Ca²⁺ channel inhibitor CoCl₂ and an intracellular Ca²⁺ chelator BAPTA completely inhibited [Ca²⁺]_i increase. CoCl₂, BAPTA and ruthenium red also inhibited disruption of (Delta) (psi) m. The results suggest that Na₂SeO₃ is able to increase [Ca²⁺]_i mitochondria permeability transition and Ca²⁺ is from extracellular Ca²⁺.

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Haitao Wang, Yafeng Liu, Zhihong Zhang, Xiangliang Yang, and Hui-Bie F. Xu "Detection of calcium by a confocal laser scanning microscope in Na₂SeO₃-treated SW480 human colonic carcinoma cells", Proc. SPIE 4536, International Workshop on Photonics and Imaging in Biology and Medicine, (12 April 2002); https://doi.org/10.1117/12.462530

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