12 April 2002 Effect of low-power HeNe laser irradiation in vitro on intracellular calcium concentration and cellular activity in mouse macrophages
Author Affiliations +
Proceedings Volume 4536, International Workshop on Photonics and Imaging in Biology and Medicine; (2002) https://doi.org/10.1117/12.462543
Event: International Workshop on Photonics and Imaging in Biology and Medicine, 2001, Wuhan, China
Abstract
Low level He-Ne laser irradiation (e.g., intravascular laser irradiation on blood, ILIB) has been widely used to treat some illness clinically. One of the action processing is by improving immunocompetence of the body, but the mechanism is still not clear. In this paper, we used mouse macrophages as study object for real-time observation of the concentration change of intracellular free calcium (abb. as [Ca2+]i) of macrophages and the distribution of the Ca2+ using LSCM (laser scanning confocal microscope) after irradiation with different dose low level He-Ne laser (the actual irradiation power (abb. as P) is 0.16 mw and the facular diameter (abb. as D) is 0.5 cm. We found interesting phenomena through the image of [Ca2+]i of macrophages. The distribution of Ca2+ in macrophages has no obvious change when the time of irradiation (abb. as Ti) is 10 min. The [Ca2+]i shows obvious increase and a concentrate phenomena in Ca2+ distribution appears when Ti reach 20 min. The change of the distribution of Ca2+ become more distinct: [Ca2+]i increase more visibly and the distribution of Ca2+ shows a spacial grads when Ti reach 30 min.
© (2002) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Chaoyang Xu, Chaoyang Xu, Shici Tan, Shici Tan, Da Xing, Da Xing, } "Effect of low-power HeNe laser irradiation in vitro on intracellular calcium concentration and cellular activity in mouse macrophages", Proc. SPIE 4536, International Workshop on Photonics and Imaging in Biology and Medicine, (12 April 2002); doi: 10.1117/12.462543; https://doi.org/10.1117/12.462543
PROCEEDINGS
5 PAGES


SHARE
Back to Top