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12 April 2002 Electrochemiluminescence immunoassay of P53 from human SWO-38 glioma cell
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Proceedings Volume 4536, International Workshop on Photonics and Imaging in Biology and Medicine; (2002) https://doi.org/10.1117/12.462550
Event: International Workshop on Photonics and Imaging in Biology and Medicine, 2001, Wuhan, China
Abstract
Electrochemiluminescence (ECL) technique for bimolecular detection offers ultra-high sensitivity and good signal-to- noise ratio. In this work, an ECL reaction and detection system were established. We used a big working electrode and a single photon counter to heighten the detection limit. The results showed that the detection limit of the system is 1/1000 fmol/L free labeled Ru(bpy)3 2+. The concentration dependence of ECL intensity was not linear in the range of low concentration (fmol/L level) free labeled Ru(bpy)3 2+. While in pmol/L level, the ECL intensity was linear. Ru(bpy)3 2+ + TPA (tripropylmine) ECL reaction system was used to indicate P53 protein from human SWO-38 cell line, which was established from grade 1-11 glioma tumor. This method could have potential applications in early-stage tumor diagnosis and environmental inspection.
© (2002) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Guihong Yan, Yonghong He, Da Xing, Yadong Wei, and Shici Tan "Electrochemiluminescence immunoassay of P53 from human SWO-38 glioma cell", Proc. SPIE 4536, International Workshop on Photonics and Imaging in Biology and Medicine, (12 April 2002); https://doi.org/10.1117/12.462550
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