12 April 2002 High-sensitivity detection of capillary electrophoresis with multiphoton excitation
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Proceedings Volume 4536, International Workshop on Photonics and Imaging in Biology and Medicine; (2002); doi: 10.1117/12.462561
Event: International Workshop on Photonics and Imaging in Biology and Medicine, 2001, Wuhan, China
Abstract
Multiphoton excited fluorescence detection is a powerful tool for probing chemistry and structure deep within biological tissue, for performing sensitive measurement on deep-UV (ex 200-300 nm) specimen. We present an approach for rapid analysis of Rhodamine B using multiphoton excited fluorescence detection coupled to capillary electrophoresis separations. In this highly versatile approach, Rhodamine B is excited through the nearly simultaneous absorption of two low-energy photons, fluorescence can be detected efficiently with low background. In these studies, Rhodamine B is fractionated in several minutes, with mass detection limits as low as 10 amol (2nM). This approach is demonstrated to be a powerful tool for analyzing the complex biological samples of minute quantities.
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Ling Fu, Qiang Lu, Hong Chen, Tongsheng Chen, Shaoqun Zeng, Qingming Luo, "High-sensitivity detection of capillary electrophoresis with multiphoton excitation", Proc. SPIE 4536, International Workshop on Photonics and Imaging in Biology and Medicine, (12 April 2002); doi: 10.1117/12.462561; http://dx.doi.org/10.1117/12.462561
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KEYWORDS
Capillaries

Fluorescence spectroscopy

Rhodamine B

Biological research

Biology

Chemistry

Deep ultraviolet

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