Continuous-flow submicroliter-scale PCR chip for DNA amplification

Wei Zheng; Shaochen Chen

doi:10.1117/12.443066

28 September 2001 Continuous-flow submicroliter-scale PCR chip for DNA amplification

Wei Zheng, Shaochen Chen

Proceedings Volume 4560, Microfluidics and BioMEMS; (2001) https://doi.org/10.1117/12.443066
Event: Micromachining and Microfabrication, 2001, San Francisco, CA, United States

Abstract

Polymerase chain reaction (PCR) is a well-described method for selective identical replication of DNA molecules. In recent years, many micromachined PCR chips have been reported. These miniaturized PCR chips have great advantages such as a significant reduction in reagent costs and vastly reduced reaction time over the conventional PCR devices. In this paper a micro analysis system that will allow submicro-liter scale, continuous-flow PCR to be conducted in a glass chip has been presented. This glass chip is achieved through thermally bonding two pyrex 7740 glass wafers. One pyrex wafer is etched to form a 20-cycle microchannel of 80 micron wide and 30 micron deep. The other pyrex wafer with microheaters is thermally bonded to the microchannel wafer to produce a closed continuous microchannel for PCR. The total length of the microchannel is 0.5 m. The size of this device is 56 mm 'e 24 mm 'e 1 mm. Three reaction temperatures are controlled by three PID controllers. This PCR chip has a significant reagent reduction with a volume of less than 1 micro-liter. With 1 micro-liter reagent, we get total reaction time of 0.5 min to 3 min depending on various flow rates. This analysis chip is fabricated using standard micromachining techniques. The advantages of this chip include small quantities of reagent needed, high throughput, rapid thermal cycling, and batch micro-fabrication resulting in a significant cost reduction.

Citation Download Citation

Wei Zheng and Shaochen Chen "Continuous-flow submicroliter-scale PCR chip for DNA amplification", Proc. SPIE 4560, Microfluidics and BioMEMS, (28 September 2001); https://doi.org/10.1117/12.443066

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available