22 February 2002 Comparative intrinsic and enhanced total photoluminescence of endospore material
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Proceedings Volume 4576, Advanced Environmental Sensing Technology II; (2002) https://doi.org/10.1117/12.456963
Event: Environmental and Industrial Sensing, 2001, Boston, MA, United States
Abstract
Two techniques are compared using total luminescence spectroscopy to detect endospore material in preparations equivalent to 3.0 x 105/ml spores. The first method applied intrinsic, steady-state photoluminescence for detection. The second approach using a binding fluorochrome derived from 4-p-dimethylaminostyrylpyridinium (DASP) to signal the presence of spore material. Comparative fluorescence emission signatures (excited at 469 nm) showed greater calibrated signal recovery (4x106 cps) for spore material at longer wavelengths using DASP. The intrinsic fluorescence emission of endospores (excited at 346 nm) occurred at shorter wavelengths and showed a reduced calibrated intensity (1.4 x 105 counts per second (cps). One major advantage of DASP appears to be its longer wavelength excitation (469 nm) that is out of the range of associated biological materials that compete for absorption at shorter UV wavelengths.
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John E. Anderson, Stanley Thomas Webb, Robert L. Fischer, Karen Kester, Clint Smith, "Comparative intrinsic and enhanced total photoluminescence of endospore material", Proc. SPIE 4576, Advanced Environmental Sensing Technology II, (22 February 2002); doi: 10.1117/12.456963; https://doi.org/10.1117/12.456963
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