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14 February 2002 Luminescent biotest for the express control of general toxicity of environment
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Proceedings Volume 4578, Fiber Optic Sensor Technology and Applications 2001; (2002)
Event: Environmental and Industrial Sensing, 2001, Boston, MA, United States
A new sensitive method based on bioinduced chemiluminescence and Daphnia as a test object is proposed for the evaluation of general toxicity of environment. The method was tested at the determination of biological toxicity of the solution of potassium chromate and at the control of water toxicity of some rivers of Ukraine. Special attention was paid to the optimization of conditions for the chemiluminescence determination. The medium of Daphnia staying was shown to have no spontaneous chemiluminescence. This was revealed using hydrogen peroxide and luminol, the optimal concentrations of which were 23 and 1.6x10-2 mmol/L. p-Iodphenol at low concentrations (4x10-5- 2x10-3 mmol/L) did not effect chemiluminescence signal but at high concentrations (4x10-2 mmol/L) an inhibition of chemiluminescence was observed. To obtain the needed intensity of chemiluminescence it is necessary to incubate no more than 5 Daphnia persons in volume of 10 mL of sample to be analyzed. The intensity of chemiluminescence of Daphnia staying medium and the sensitivity of this organism to potassium chromate increased at the temperature increasing from 24 to 32 degree(s)C. Medium of Daphnia staying can be preserved in refrigerator for several hours without loss of chemiluminescence signal.
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Nickolaj F. Starodub, Inna A. Levkovetz, Svetlana P. Ivashkevich, Valentyna M. Starodub, and Vladimir I. Nazarenko "Luminescent biotest for the express control of general toxicity of environment", Proc. SPIE 4578, Fiber Optic Sensor Technology and Applications 2001, (14 February 2002);

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