Diagnosis of oral tumors using light-induced autofluorescence spectra at multiple excitation wavelengths

Wei Zheng; Malini Olivo; K. Chee Soo

doi:10.1117/12.446649

25 October 2001 Diagnosis of oral tumors using light-induced autofluorescence spectra at multiple excitation wavelengths

Wei Zheng, Malini Olivo, K. Chee Soo

Author Affiliations +

Proceedings Volume 4597, Biophotonics Instrumentation and Analysis; (2001) https://doi.org/10.1117/12.446649
Event: International Symposium on Photonics and Applications, 2001, Singapore, Singapore

Abstract

Fluorescence excitation-emission matrices (EEMs) were measured from normal and tumourous oral tissues in vitro using excitation wavelengths ranging from 220 to 500 nm. The prominent fluorescence excitation-emission peaks: (228 nm; 260/338/475 nm), (282 nm; 337/475/660 nm), (335 nm; 472 nm) and (465 nm, 523 nm) for normal tissues; (228 nm; 335/475/660 nm), (282 nm; 330/660 nm) and (350 nm; 460 nm) for tumour tissues, can be found in EEM maps, indicating the dominant fluorophores responsible for tissue autofluorescence are tryptophan, NADH, FAD, and porphyrin compounds in oral tissues. The optimal excitation wavelengths were found to be 280, 335, and 465 nm. The empirical discrimination algorithms based on I₄₇₅/I₆₆₀, and I₅₂₀/I₆₆₀ were developed. These algorithms yield an average sensitivity and specificity of 95% and 88%, respectively.

Citation Download Citation

Wei Zheng, Malini Olivo, and K. Chee Soo "Diagnosis of oral tumors using light-induced autofluorescence spectra at multiple excitation wavelengths", Proc. SPIE 4597, Biophotonics Instrumentation and Analysis, (25 October 2001); https://doi.org/10.1117/12.446649

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