25 October 2001 Fluorescence micro-imaging of living cells and biomolecules with ultrahigh NA objectives
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Proceedings Volume 4597, Biophotonics Instrumentation and Analysis; (2001) https://doi.org/10.1117/12.446647
Event: International Symposium on Photonics and Applications, 2001, Singapore, Singapore
Abstract
Optical imaging with a high detecting power is very instrumental to dynamic observations of bio-molecular objects in water. To pioneer this field of imaging researches further, we used novel objective lenses of 1.65 and 1.45 in NA comparatively with 1.35 NA lens. The lenses of ultra high NA have a high resolving power and very useful properties for observation of high contrast images of fluorescently labeled molecules and subcellular organelles including DNA and membrane fusion proteins.
© (2001) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Susumu Terakawa, Susumu Terakawa, Takashi Tsuboi, Takashi Tsuboi, Takashi Sakurai, Takashi Sakurai, Andreas Jeromin, Andreas Jeromin, Yoshihiko Wakazono, Yoshihiko Wakazono, Seiji Yamamoto, Seiji Yamamoto, Katsuyuki Abe, Katsuyuki Abe, } "Fluorescence micro-imaging of living cells and biomolecules with ultrahigh NA objectives", Proc. SPIE 4597, Biophotonics Instrumentation and Analysis, (25 October 2001); doi: 10.1117/12.446647; https://doi.org/10.1117/12.446647
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