15 October 2001 Fabrication of polyurethane molecular stamps for the synthesis of DNA microarray
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Proceedings Volume 4601, Micromachining and Microfabrication Process Technology and Devices; (2001) https://doi.org/10.1117/12.444752
Event: International Symposium on Optoelectonics and Microelectronics, 2001, Nanjing, China
Abstract
Polyurethane based on polypropylene glycol (PPG) and Toluene diisocyanate (TDI) using 3,3'-dichloride-4,4'- methylenedianiline (MOCA) as the crosslinker is presented for the first time to fabricate molecular stamps (PU stamps) for the synthesis of DNA microarray with contact procedure. The predictability of the process is achieved by utilizing commercially available starting materials. SEM analysis of the morphology of PU stamps and master showed that PU elastometer could replicate subtly the motherboard's patterns with high fidelity. It was proved from the contact angle measurement that PU stamps surface has good affinity with acetonitrile, which guarantee the well-distribution of DNA monomers on patterned stamps. Laser confocal fluorescence microscopy images of oligonucleotide arrays confirmed polyurethane is an excellent material for molecular stamps.
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Zhengchun Liu, Zhengchun Liu, Quanguo He, Quanguo He, Pengfeng Xiao, Pengfeng Xiao, Nongyao He, Nongyao He, Zuhong Lu, Zuhong Lu, Liang Bo, Liang Bo, } "Fabrication of polyurethane molecular stamps for the synthesis of DNA microarray", Proc. SPIE 4601, Micromachining and Microfabrication Process Technology and Devices, (15 October 2001); doi: 10.1117/12.444752; https://doi.org/10.1117/12.444752
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