Optical properties of cytostatic drugs used in cancer treatment

Mihail-Lucian Pascu; Ioan Mogos; Mironel Enescu; Angela Staicu; Sorina Truica; Letitia Voicu; Doina M. Gazdaru; Mihaela Oana Pascu; Alina Radu

doi:10.1117/12.446699

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23 October 2001 Optical properties of cytostatic drugs used in cancer treatment

Mihail-Lucian Pascu, Ioan Mogos, Mironel Enescu, Angela Staicu, Sorina Truica, Letitia Voicu, Doina M. Gazdaru, Mihaela Oana Pascu, Alina Radu

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Proceedings Volume 4606, Laser Florence 2000: A Window on the Laser Medicine World; (2001) https://doi.org/10.1117/12.446699
Event: Laser Florence 2000: A Window on the Laser Medicine World, 2000, Florence, Italy

Abstract

A spectroscopical characterization of methotrexate, cytostatic drug used frequently in cancer therapy, was performed. The absorption, emission and excitation spectra were measured for methotrexate solutions in natural saline and sodium hydroxide at concentration in the range 10^-5 M -10^-6 M and pH 8.4. The absorption bands are noticed in the spectral range 250 nm - 450 nm. The fluorescence excitation was made at 340 nm and 370 nm; the fluorescence emission was detected in the spectral range 400 nm - 500 nm with a maximum at 450 nm. The behavior of absorption and fluorescence spectra of methotrexate solution exposed to uv-visible light was investigated. The irradiation was made using an Xe lamp (emission between 325 nm and 420 nm and power density of 11 mW/cm²). The exposure time was between 15 min. and 3 h. Major modifications on absorption bands for irradiation times longer than 1 hour were observed. Furthermore, the methotrexate solutions become strongly fluorescent after irradiation. The observed changes are not linear with the exposure time indicating complex photochemical processes which implies, at least, one intermediate product.

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Mihail-Lucian Pascu, Ioan Mogos, Mironel Enescu, Angela Staicu, Sorina Truica, Letitia Voicu, Doina M. Gazdaru, Mihaela Oana Pascu, and Alina Radu "Optical properties of cytostatic drugs used in cancer treatment", Proc. SPIE 4606, Laser Florence 2000: A Window on the Laser Medicine World, (23 October 2001); https://doi.org/10.1117/12.446699

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