Concentration measurement of lysosome enzymes in blood by fluorimetric analysis method

Marina M. Strinadko; Elena M. Strinadko

doi:10.1117/12.455227

5 February 2002 Concentration measurement of lysosome enzymes in blood by fluorimetric analysis method

Marina M. Strinadko, Elena M. Strinadko

Proceedings Volume 4607, Selected Papers from Fifth International Conference on Correlation Optics; (2002) https://doi.org/10.1117/12.455227
Event: Fifth International Conference on Correlation Optics, 2001, Chernivsti, Ukraine

Abstract

The diagnostics of heritable disease series and sugar diabetes, myocardial infarction, collagenosis and kidney diseases widely uses the measurement of lysosomic enzymes in blood. In the present research work the definition procedure of concentration (beta) -glucuronidase with the help of fluorimetric analysis is offered, which allows using microamounts of biological fluids and samples with low enzyme activity which is especially important in paediatric practice. Due to the sharp sensibility of fluorimetric analysis and high speed of luminescent reactions the procedure gives an opportunity to obtain the result in the minimum terms as well as the use of small amounts of reaction mixture. The incubation in large dilution leads thereby to the elimination of influence of endogenic inhibitors and activators.

Citation Download Citation

Marina M. Strinadko and Elena M. Strinadko "Concentration measurement of lysosome enzymes in blood by fluorimetric analysis method", Proc. SPIE 4607, Selected Papers from Fifth International Conference on Correlation Optics, (5 February 2002); https://doi.org/10.1117/12.455227

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