An in vivo study was conducted to investigate the feasibility, mechanical function, and chronic biocompatibility of a new range of light-activated surgical adhesives for vascular anastomosis. Porcine carotid arteries (n=12) and femoral arteries (n=12) were exposed, and a 0.3 -0.6cm longitudinal incision was made in the arterial walls. The vessels were divided equally into two groups. Vessels belonging to the first group were repaired using a surgical adhesive, composed of a poly(L-lactic-co-glycolic acid) scaffold doped with the traditional protein solder mix of serum albumin and indocyanine green dye mixed in deionized water. The adhesive was applied across the incision and denatured using an 805-nm diode laser. Vessels belonging to the second group formed part of a control study, and were repaired using conventional suturing techniques. Blood flow was restored to the vessels immediately after the procedure and the incision sites were checked for patency. The strength and hemostatic abilities of the new surgical adhesives were evaluated in the context of arterial pressure, persistence of hemostasis and presence of any inflammatory reaction after 3 days. The adhesive technique compared favorably with the suture technique. Repairs formed with the adhesive technique were achieved more rapidly than suturing, and acute leakage was observed less frequently. Repairs closed by suture did not burst, but leaked at pressures significantly below those of vessels closed with the adhesive material. Finally, the adhesive technique produced better histology than the suture technique, suggesting that it holds great promise as an alternative to suturing.