In-vivo characterization of endogenous porphyrin fluorescence from DMBA-treated Swiss Albino mice skin carcinogenesis for measuring tissue transformation

Singaravelu Ganesan; Jeyasingh Ebenezar; Srinivasan Hemamalini; Prakasa Rao Aruna

doi:10.1117/12.465237

1 May 2002 In-vivo characterization of endogenous porphyrin fluorescence from DMBA-treated Swiss Albino mice skin carcinogenesis for measuring tissue transformation

Singaravelu Ganesan, Jeyasingh Ebenezar, Srinivasan Hemamalini, Prakasa Rao Aruna

Author Affiliations +

Proceedings Volume 4613, Optical Biopsy IV; (2002) https://doi.org/10.1117/12.465237
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Steady state fluorescence spectroscopic characterization of endogenous porphyrin emission from DMBA treated skin carcinogenesis in Swiss albino mice was carried out. The emission of endogenous porphyrin from normal and abnormal skin tissues was studied both in the presence and absence of exogenous ALA to compare the resultant porphyrin emission characterictics. The mice skin is excited at 405nm and emission spectra are scanned from 430 to 700nm. The average fluorescence emission spectra of mice skin at normal and various tissues transformation conditions were found to be different. Two peaks around 460nm and 636nm were observed and they may be attributed to NADH, Elastin and collagen combination and endogenous porphyrin emission. The intensity at 636nm increases as the stage of the cancer increases. Although exogenous ALA enhances the PPIX level in tumor, the synthesis of PPIX was also found in normal surrounding skin, in fact, with higher concentration than that of tumor tissues.

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Singaravelu Ganesan, Jeyasingh Ebenezar, Srinivasan Hemamalini, and Prakasa Rao Aruna "In-vivo characterization of endogenous porphyrin fluorescence from DMBA-treated Swiss Albino mice skin carcinogenesis for measuring tissue transformation", Proc. SPIE 4613, Optical Biopsy IV, (1 May 2002); https://doi.org/10.1117/12.465237

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