1 May 2002 Ultraviolet emission and excitation fluorescence spectroscopic characterization of DMBA-treated Swiss Albino mice skin carcinogenesis for measuring tissue transformation
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Proceedings Volume 4613, Optical Biopsy IV; (2002) https://doi.org/10.1117/12.465227
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States
Abstract
The ultraviolet fluorescence emission spectra of skin tissues under different pathological conditions were measured at 280nm excitation. At this excitation wavelength, the normal skin showed a primary peak emission at 352nm and this primary peak emission from neoplastic skin shows a blue shift with respect to normal tissue. This blue shift increases as the stage of abnormality increases and it is maximum (19nm) for well-differentiated squamous cell carcinoma. This alteration is further confirmed from fluorescence excitation spectra of the tissues for 340nm emission. The study concludes that the change in the emission of tryptophan around 340nm may be due to partial unfolding of protein.
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Prakasa Rao Aruna, Prakasa Rao Aruna, Srinivasan Hemamalini, Srinivasan Hemamalini, Jeyasingh Ebenezar, Jeyasingh Ebenezar, Singaravelu Ganesan, Singaravelu Ganesan, } "Ultraviolet emission and excitation fluorescence spectroscopic characterization of DMBA-treated Swiss Albino mice skin carcinogenesis for measuring tissue transformation", Proc. SPIE 4613, Optical Biopsy IV, (1 May 2002); doi: 10.1117/12.465227; https://doi.org/10.1117/12.465227
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