Ultraviolet emission and excitation fluorescence spectroscopic characterization of DMBA-treated Swiss Albino mice skin carcinogenesis for measuring tissue transformation

Prakasa Rao Aruna; Srinivasan Hemamalini; Jeyasingh Ebenezar; Singaravelu Ganesan

doi:10.1117/12.465227

1 May 2002 Ultraviolet emission and excitation fluorescence spectroscopic characterization of DMBA-treated Swiss Albino mice skin carcinogenesis for measuring tissue transformation

Prakasa Rao Aruna, Srinivasan Hemamalini, Jeyasingh Ebenezar, Singaravelu Ganesan

Author Affiliations +

Proceedings Volume 4613, Optical Biopsy IV; (2002) https://doi.org/10.1117/12.465227
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

The ultraviolet fluorescence emission spectra of skin tissues under different pathological conditions were measured at 280nm excitation. At this excitation wavelength, the normal skin showed a primary peak emission at 352nm and this primary peak emission from neoplastic skin shows a blue shift with respect to normal tissue. This blue shift increases as the stage of abnormality increases and it is maximum (19nm) for well-differentiated squamous cell carcinoma. This alteration is further confirmed from fluorescence excitation spectra of the tissues for 340nm emission. The study concludes that the change in the emission of tryptophan around 340nm may be due to partial unfolding of protein.

Citation Download Citation

Prakasa Rao Aruna, Srinivasan Hemamalini, Jeyasingh Ebenezar, and Singaravelu Ganesan "Ultraviolet emission and excitation fluorescence spectroscopic characterization of DMBA-treated Swiss Albino mice skin carcinogenesis for measuring tissue transformation", Proc. SPIE 4613, Optical Biopsy IV, (1 May 2002); https://doi.org/10.1117/12.465227

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