Application of Fourier-transform infrared ellipsometry to quantify bioloical molecules in animal tissues

Enric Garcia-Caurel; Bernard Drevillon; Laurent Schwartz

doi:10.1117/12.460790

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27 March 2002 Application of Fourier-transform infrared ellipsometry to quantify bioloical molecules in animal tissues

Enric Garcia-Caurel; Bernard Drevillon; Laurent Schwartz

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Proceedings Volume 4614, Biomedical Vibrational Spectroscopy II; (2002) https://doi.org/10.1117/12.460790
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Immunohistochemistry contributes to determine the site of origin of the tumor, helps setting the treatment and the prognosis. However, these assays are both time consuming when treatment may be an emergency, and expensive. Instead of the usual biochemistry we are using for the first time for medical issues Fourier-transform IR ellipsometry, a non- invasive optical characterization technique used in the semiconductor field to characterize bare substrates and thin films. Working in the mid-IR makes this technique sensitive to the molecules present inside the cell. Since the ellipsometric analysis can be performed on tissue slides extended on a specular surface, they may provide the physician with a chemical map of the sample. Here we show that ellipsometry can detect and measure physiological concentration of glycogen diluted in water and retrieve the polysaccharide band in a tissue slide. Our objective is to show that ellipsometry can give a quick and comprehensive analysis of the diverse biological markers forming the living matter.

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Enric Garcia-Caurel, Bernard Drevillon, and Laurent Schwartz "Application of Fourier-transform infrared ellipsometry to quantify bioloical molecules in animal tissues", Proc. SPIE 4614, Biomedical Vibrational Spectroscopy II, (27 March 2002); https://doi.org/10.1117/12.460790

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