27 March 2002 Skin hydration by spectroscopic imaging using multiple near-infrared bands
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Proceedings Volume 4614, Biomedical Vibrational Spectroscopy II; (2002) https://doi.org/10.1117/12.460783
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States
Near-infrared spectroscopic methods have been developed to determine the degree of hydration of human skin in vivo. Reflectance spectroscopic imaging was used to investigate the distribution of skin moisture as a function of location. A human study in a clinical setting has generated quantitative data showing the effects of a drying agent and a moisturizer on delineated regions of the forearms of eight volunteers. Two digital imaging systems equipped with liquid-crystal tunable filters were used to collect stacks of monochromatic images at 10-nm intervals over the wavelength bands 650-1050 nm and 960-1700 nm. Images generated from measurements of water absorption-band areas at three different near-IR wavelengths (970, 1200, and 1450 nm) showed obvious differences in the apparent distribution of water in skin. Changes resulting from the skin treatments were much more evident in the 1200-nm and 1450-nm images than in the 970-nm ones. The variable sensitivity of the method at different wavelengths has been interpreted as being the result of different penetration depths of the infrared light used in the reflectance studies. Ex-vivo experiments with pigskin have provided evidence supporting the relationship between wavelength and penetration depth. Combining the hydration results from several near-IR water bands allows additional information on hydration depth to be obtained.
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E. Michael Attas, Michael G. Sowa, Trevor B. Posthumus, Bernhard J. Schattka, Henry H. Mantsch, Shuliang L. Zhang, "Skin hydration by spectroscopic imaging using multiple near-infrared bands", Proc. SPIE 4614, Biomedical Vibrational Spectroscopy II, (27 March 2002); doi: 10.1117/12.460783; https://doi.org/10.1117/12.460783

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