Photothermal response of live cell depends upon cell metabolic state

Dmitry Lapotko; Tat'yana Romanovskaya; Elena Gordiyko

doi:10.1117/12.469854

10 June 2002 Photothermal response of live cell depends upon cell metabolic state

Dmitry Lapotko, Tat'yana Romanovskaya, Elena Gordiyko

Proceedings Volume 4618, Biomedical Optoacoustics III; (2002) https://doi.org/10.1117/12.469854
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Photoacoustic and photothermal (PT) methods in live cells studies allow to realize direct monitoring as they do not require cell pretreatment with chemicals and because the sources of output signal are cell chromophores. The origin of PT signals from single cells that are irradiated with short pump laser pulse was studied experimentally under different rates of cell metabolism. The cells in different metabolic status were found to produce PT response of specific shape that occurs in specific pump energy region for each pump wavelength. This type of PT response is associated with secondary phenomena that are induced in cells by thermal impact. An ability of live cell to produce such PT response was found to depend upon the redox state of cell respiratory chain (RC) components that absorb pump laser radiation. The results of PT studies of in vitro modulation of hepatocyte metabolic state are presented as function of RC state (reduced/oxidized) and laser parameters (wavelength and energy). Obtained results show that several parameters of laser-cell interaction depend upon RC redox state: shape and amplitude of PT response from single cell, energy threshold for laser damage to cell.

Citation Download Citation

Dmitry Lapotko, Tat'yana Romanovskaya, and Elena Gordiyko "Photothermal response of live cell depends upon cell metabolic state", Proc. SPIE 4618, Biomedical Optoacoustics III, (10 June 2002); https://doi.org/10.1117/12.469854

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available