14 June 2002 Murine myocardium OCT imaging with a blood substitute
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Proceedings Volume 4619, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications VI; (2002) https://doi.org/10.1117/12.470479
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States
Abstract
Imaging of the in vivo murine myocardium using optical coherence tomography (OCT) is described. Application of conventional techniques (e.g. MRI, Ultrasound imaging) for imaging the murine myocardium is problematic because the wall thickness is less than 1.5mm (20g mouse), and the heart rate can be as high as six-hundred beats per minute. To acquire a real-time image of the murine myocardium, OCT can provide sufficient spatial resolution (10 micrometers ) and imaging speed (1000 A-Scans/s). Strong light scattering by blood in the heart causes significant light attenuation making delineation of the endocardium-chamber boundary problematic. By replacing whole blood in the mouse with an artificial blood substitute we demonstrate significant reduction of light scattering in the murine myocardium. The results indicate a significant reduction in light scattering as whole blood hematocrit is diminished below 5%. To measure thickness change of the myocardium during one cycle, a myocardium edge detection algorithm is developed and demonstrated.
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Jeehyun Kim, Joseph W. Villard, Ho Lee, Marc D. Feldman, Thomas E. Milner, "Murine myocardium OCT imaging with a blood substitute", Proc. SPIE 4619, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications VI, (14 June 2002); doi: 10.1117/12.470479; https://doi.org/10.1117/12.470479
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